"I didn't feel I was being discriminatory. The woman was with a bloke, who could see perfectly well, and there was no reason for the dog to be in the pub.
"It didn't look like a guide dog as it had no harness or collar on it, and I wasn't convinced that it was.
"I've been in this business for 21 years and I've nothing against dogs, and guide dogs have been in here before."
The Disability Rights Commission, an independent body established to stop discrimination and promote equality of opportunity for disabled people, has advised her to write to the landlord about the incident.
If he did not respond within 14 days or she was not satisfied with his reply, they would take up the case on her behalf.
The Disability Discrimination Act 1995 established legal rights for disabled people, covering employment, access to services, education, transport and housing. New employment rights and rights of access became law in October, 2004.
RICHARD McLINDEN, access officer for the Liverpool Association of Disabled People, said refusing to serve someone for bringing a guide dog into the pub was illegal.
He said: "You can't do that under the Disability Discrimination Act.
"If it was a wheelchair user would they have asked her to take that outside? The dog is her eyes as the wheelchair is someone's legs.
"This is illegal and it's stupidity. They would not have asked David Blunkett to take his dog outside.
"Whether she had someone with her makes no difference. The Act is to make people independent and people should be trained in it.
"What if she had to go to the toilet or her friend had to leave early?"
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