Despite knowing nothing about brewing, within three years of taking over the 150-year-old Robert Cains & Co in 2002, the brothers increased production fourfold, and more than doubled employment to 97.
The brewery had been losing around £2m a year and was on the verge of collapse.
The entrepreneurs, who grew up on a council estate in Kent and made their money in fish and chip shops, have since picked up a number of personal accolades for turning round the fortunes of the brewery and also ensured that Cains beers have secured several industry awards for quality and innovation.
Their deal is the latest set up by the Liverpool Culture Company, which is aiming to raise about £40m from backers to help fund the next three years of showpiece celebrations.
Sponsors so far include NWDA, Hill Dickenson, United Utilities and Radio City.
Councillor Mike Storey, leader of Liverpool City Council, said: "The Dusanj brothers epitomise the new confidence flowing in Liverpool's business community. They have rejuvenated Cains and for them to be part of Liverpool 08 will be a marriage of two fantastic success stories."
A recent business survey found almost a third of Liverpool businesses are planning to invest in their company ahead of 2008 and 20% plan to take on an average of four extra staff.
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